Nvysis alk break apart fish probe kit pdf files

Breakapart fluorescence in situ hybridization fish is the gold standard test. The prices for the alk break apart fish probe kit ivd and the. Automated fish analysis using dualfusion and breakapart. We stock all major equipment types such as spectrum analyzers, signal generators, oscilloscopes, power meters, logic. Visualizing fish hybridization evaluate hybridized ffpe nsclc tissue specimen. Vysis alk break apart fish probe kit ivd, 20 assays, 06n38033, 00884999042728. Ros1 rearrangements were identified in 16 patients with nsclc using fish. Agilent technologies 89410a operators manual pdf download. The alk fish probe set is designed to detect rearrangements involving portions of the human alk gene located on chromosome. Everybody wants to blame the probe for their bod problems, especially for problems with dilution water blanks. The kit contains two differentially labeled locus specific probes lsp, one covering the 5 start portion of the alk gene and. In addition to revealing breaks, which can lead to translocation of parts of the gene, inversion, or its fusion to other genes, the probe set can also be used to identify. Fluorescenceinsituhybridization,immunohistochemistry,and next. The probes are repeatfree, able to target specific sequences of interest, and use highfidelity oligos designed in silico.

The ros1 6q22 break apart fish probe is designed to detect a chromosomal rearrangement involving the ros1 gene located on chromosome 6q22. Ros1 6q22 break apart fish probe orangegreen biocare. Fluorescence in situ hybridization fish for detecting anaplastic. Akt1cen14q fish probe akt2cen19p fish probe akt3cen1q fish probe alk cen2p fish probe amycen1q fish probe apccen5q fish probe aprtcen16q fish probe representative images of normal human cell lymphocyte stain with the dual color fish probe. Some of the most commonly required parts are listed on this page. Clinicopathologic analysis of ros1rearranged nonsmallcell.

Fluorescence in situ hybridization fish was performed with the following probes. Can i automatically stop my infiniium from acquiring new waveforms after a certain period of time or number of triggers. Jul 24, 2012 although fish can be performed on ffpe tumour specimens and detect multiple alk fusion variants, there are various challenges related to the fish technique, e. Fluorescenceinsituhybridization,immunohistochemistry,and. Mll break apart dnafish probe two color, break apart. Of the 12 alk positive cases, hybridization with vysis alk ros1 dual break apart probe kit was successful in 10 cases success rate 1012, 83% and of these 10 cases, all showed alk rearrangement 100% concordance with the results of vysis alk break apart fish probe kit. In addition to revealing breaks, which can lead to translocation of parts of the gene, inversion, or its fusion to other genes, the probe set can also be used to identify other tfeb aberrations such as deletions or.

Alk break apart fish probe kit assay for detecting alk nsclc. Alk inversion, locusspecific identifier dualcolor breakapart probes vysis. Vysis alk break apart fish probe kit ce, 20 assays, 06n38023, 00884999042766. The majority of fish probes available on the market use bac technology. The vysis fish chromosome search tool provides the most uptodate vysis fish probe information. Some pcr targets are problematic to amplify due to their content or structure. The resultant fusion proteins generated from alk gene rearrangement plays a vital role in driving the pathogenesis of several different types of cancer, such as.

The alk fish probe set is designed to detect rearrangements involving portions of the human alk gene located on chromosome band 2p23. Theyre designed to flank either side of a gene, so that in the event of a translocation, the two colors will split. Herculase ii fusion dna polymerase provides you with superior yields in both routine and challenging pcr applications, with the additional benefit of allowing the use of fast cycling times. Keysight n2893a 100 mhz current probe users guide introduction the n2893a is a wideband, dc to 100 mhz, active current probe. Fluorescence in situ hybridization fish preparation of fish probe. Alk break apart dnafish probe two color, break apart probe. Probe description the spec ros1 dual color break apart probe is a mixture of two direct labeled probes hybridizing to the 6q22.

The tfeb break apart fish probe kit is designed to detect rearrangements in the human tfeb gene located on chromosome band 6p21. Agilent technologies 54642d manuals manuals and user guides for agilent technologies 54642d. The probe features low noise and low circuit insertion loss. Probe description the spec erg dual color break apart probe is a mixture of two direct labeled probes hybridizing to the long arm of chromosome 21. N2793a 800 mhz differential probe n279368700 differential probe accessory kit for n2819an2793a including 2 each pincer clip, 2 each micro ic clip, 2 each extension lead 0. We have 5 agilent technologies 54642d manuals available for free pdf download. We stock a large inventory of cessna replacement parts.

View and download agilent technologies 89410a operators manual online. User manual, service manual, programmers manual, datasheet. This kit contains a mixture of lsimyc spectrumorange probe and lsimyc spectrumgreen probe. Alk break apart dnafish probe two color, break apart. Edward burtons lab contains the insert dat and is published in neuroscience. Ros1negative cases showed two fused yellow signals or closely apposed green and red signals. This probe is not for electrically insulating the circuit under test and the measuring instrument. Ros1 rearrangement, bronchoalveolar carcinoma clone corresponding to the 5.

Braf break apart fish probe kit introduction the braf break apart fish probe kit is designed to detect rearrangements in the human braf gene located on chromosome band 7q34. A fish test that detects deletion of the lsi tp53, lsi atm, and lsi ds319 probe targets and gain of the d12z3 sequence. A fish test that detects rearrangements involving the alk gene in nonsmall cell lung cancer tissue specimens. Alk break apart dnafish probe two color, break apart probe h21002 vc s20ocwf standard fish procedure note. Materials provided if you receive reagents that are in a condition contrary to label vysis alk break apart fish probe kit list no. Fluorescence in situ hybridization fish is a technique that allows the the vysis alk break apart fish probe kit is a qualitative test to visualization of specific chromosome nucleic acid sequences within a detect rearrangements involving the alk gene via fluorescence in situ cellular preparation. Clinicopathologic analysis of ros1rearranged nonsmall. This probe is intended for methanolaceticacid fixed cells and tissue sections. The e2697a includes one 10073c general purpose 100 mhz, 10. Eml4alk testing in nonsmall cell carcinomas of the lung. We can supply the agilent hp n3302a wireless connectivity test set. Fish for alk background information the food and drug administrations august 2011 approval of the targeted therapy crizotinib xalkori, pfizer, new york, n.

Simultaneously, the fda approved the vysis alk break apart fluorescence in situ hybridization fish probe kit abbott molecular as a. Vysis alk break apart fish probe kit molecular catalog. Empire genomics dualcolored breakapart fish probes have helped thousands of clinicians and researchers quickly and reliably track gene translocations. Do not reconstitute or dilute with hybridization buffer.

The aim of this study was to investigate an automated fluorescent in situ hybridization analysis method using grid sampling on tissue sections, and compare the performance of dualfusion df and. The left image is chromosomes at metaphase, and the right image is an interphase nucleus. Vysis alk break apart fish probe kit en food and drug. Vysis alk break apart fish probe kit abbott molecular contact. Microcost analysis of alk rearrangement testing by fish to. Alk 59 probe spectrumgreen and the lsi alk 39 probe spectrumorange were applied, hybridized, and assessed along with standard controls.

N2792an2818a 200 mhz and n2793an2819a 800 mhz differential. The resultant fusion proteins generated from alk gene rearrangement plays a vital role in driving the pathogenesis of several different types of cancer, such. March 2014agilent technologies introduced surefish alk, ros1, and ret breakapart translocation probes, expanding its offering of oligonucleotidebased fluorescent in situ hybridization products. The kit contains two differentially labeled locus specific probes lsp, one covering the 5 start portion of the alk gene and some adjacent genomic sequence, the other covering the 3 end part and the. Request pdf high prevalence of immunoglobulin light chain gene aberrations as revealed by fish in multiple myeloma and mgus multiple myeloma mm is a malignant bcell neoplasm characterized. Validation of breakapart and fusion myc probes using a. Vysis alk break apart fish probe kit abbott molecular. Ros1 rearrangements using fluorescence in situ hybridization might be a helpful tool for the identification of patients likely to respond to ros1 kinase targeting therapies. The vysis alk break apart fish probe kit is a qualitative test to detect rearrangements involving the alk gene via fluorescence in situ hybridization fish in formalinfixed paraffinembedded ffpe nonsmall cell lung cancer nsclc tissue specimens to aid in identifying those patients eligible for treatment with xalkori crizotinib. Intended use the myc break apart dnafish probe is designed to detect the translocation between the myc gene located at 8q24 and one of 11 known translocation partner loci using fluorescence in situ hybridization fish. This patent pending, interactive tool provides singleclick access from fish probe descriptions directly to fish probe maps, hybridization images, and associated product page and ordering information.

Xl alk ba is a break apart probe with an orange labeled probe hybridizing to the proximal region within alk in 2p23 and a green labeled probe hybridizing distal to alk. Agilent basic dc power supplies offer essential features for a tight budget. The vysis alk break apart fish probe kit is shipped on dry ice. The 8q24 myc rearrangement is detected using the vysis lsimyc dual color breakapart rearrangement probe kit abbott laboratories, des plaines, il usa. Probe map details based on ucsc genome browser grch37hg19. The alk gene can fuse with one of 20 known alk gene rearrangement partners, the most common being t2. Rp111036c2 ends was kindly provided by professor anthony john iafrate 6. Agilent hp 1141a differential probe and 1142a probe.

Caution this probe is designed to perform differential measurements between two points on the circuit under test. Asr analyte specific reagent analytical and performance characteristics are not established gpr general purpose reagent ivd in vitro diagnostics rx only ruo research use only. Note that eml4 is normally on the opposite strand to alk, and that both probes on the alk gene red orange and green are close together, explaining. Vysis alk break apart fishsondenkit abbott molecular. Akt3cen1q fish probe alkcen2p fish probe amycen1q fish probe apccen5q fish probe aprtcen16q fish probe representative images of normal human cell lymphocyte stain with the dual color fish probe. Ros1 break apart fish probe kit introduction the ros1 break apart fish probe kit is designed to detect rearrangements in the human ros1 gene located on chromosome band 6q22. Mll break apart dnafish probe two color, break apart probe h11002 vc s20ocwf standard fish procedure note. In addition to revealing breaks, which can lead to translocation of parts of the gene, inversion, or its fusion to other genes. The 1141a1142a probe system allows measurement of small differential signals in the presence of much larger commonmode signals. The probes are repeatfree, able to target specific sequences. N2893a probe n2893a users guide 7 danger take the following precautions to ensure that the infiniium does not form a bridge between the probe and any hazardous live part. In ros1positive patients n 16, a split pattern was observed in 14 tumors 87. At least 50 nonoverlapping nuclei were analyzed, and the localization of the lsi alk 59 probe green and lsi alk 39 probe orange signals were recorded and interpreted according to the manufacturers. The orange fluorochrome direct labeled probe hybridizes at 21q22.

The following procedure will allow you to check your own probe without sending it out to anybody. Ros1 6q22 break apart fish probe orangegreen biocare medical. Isolate the autoprobe interface to which the prob e is connected from other autoprobe interfaces. The input voltage should not exceed the input voltage rating of the measuring instrument. Validation of alkros1 dual break apart fish probe probe. The single output, 120200 w, gpib, e3632ae3634a provide dual range outputs. High prevalence of immunoglobulin light chain gene. Mll break apart dnafish probe two color, break apart probe. The keysight 10073c passive probe is our most rugged 500 mhz probe. Keysightagilent hp 1141a differential probe and power. It also features the autoprobe interface which makes current measurements as simple as those made with active voltage probes.

Only a technologist familiar with cytogenetic methods and trained for the fish technique can perform the assay. Thermocouplesuniversal replacement probes california power. Agilent expands catalog of surefish breakapart probes. The alk break apart dnafish probe is designed to detect the translocation between the alk gene located at 2p23 and one of at least 14 known translocation partner loci using fluorescence in situ hybridization fish. Chromosomal rearrangements involving ros1 have been implicated in multiple cancer indications1,2 and has been identified as an oncogenic driver in nonsmall cell lung cancer3. The ros1 gene encodes a receptor tyrosine kinase receptor. Agilent 5462061601 logic analyzer probe cable 16 channels, input z. If you require cessna parts not listed here, please provide us with the cessna part numbers and we will provide you with a prompt quotation.

Vysis alk break apart fish probe kit hybridization conditions may be adversely affected by the use of 1. Fivemicron unstained ffpe sections were used for fish analysis. Myc break apart dnafish probe myc c empowering personalized. Thermocouplesuniversal replacement probes universal replacement probes egt probe, type k, style 86255 pn mmm111 cht probe, type j, style an55411 pn an55411 cht probe, type k pn cht510 cessna cht probe pn s23343. Vysis lsi alk dual color break apart fish probe reagents other than those provided by abbott molecular. Single, dual, and variable pitch signal pins insert the single or dual signal pins into the probe.